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Clinical Chemistry 35: 141-143, 1989;
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Clinical Chemistry, Vol 35, 141-143, Copyright © 1989 by American Association for Clinical Chemistry

Turbidimetric latex immunoassay of placental lactogen on microtiter plates

D Collet-Cassart, JN Limet, L Van Krieken and R De Hertogh
Unit of Experimental Medicine, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.

In this latex immunoassay for human placental lactogen, microtiter plates are used as the reaction vessel and the absorbance at 405 nm is measured to quantify the reactions. This 30-min assay necessitates only one serum dilution and two pipetting steps. The calibration curve extends from 0.5 to 15 mg/L. CVs range from 4.2% to 7.0% for within-run determination and from 7.0% to 11.2% for between-run determinations. A correlation coefficient of 0.949 was obtained for 84 sera when the method was compared with a commercial radioimmunoassay.


The following articles in journals at HighWire Press have cited this article:


Home page
 Annals of Clinical & Laboratory ScienceHome page
T. Makino
Use of Microtiter Plates for Latex Agglutination Immunoassay of Serum Alpha2-Macroglobulin
Ann. Clin. Lab. Sci., April 1, 2001; 31(2): 205 - 210.
[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the American Association for Clinical Chemistry.