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Clinical Chemistry 35: 74-76, 1989;
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Clinical Chemistry, Vol 35, 74-76, Copyright © 1989 by American Association for Clinical Chemistry

Preparation of fecal samples for assay of volatile fatty acids by gas- liquid chromatography and high-performance liquid chromatography

HM Chen and CH Lifschitz
USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030.

We describe a procedure for preparing fecal samples for determination of volatile fatty acids (VFAs) by gas-liquid chromatography (GLC) and "high-performance" liquid chromatography (HPLC). The simple, one-step procedure involves only ultrafiltration through a membrane with a molecular-mass cutoff of 3000 Da. As revealed by the GLC chromatograms, ultrafiltration appears to be as effective as steam distillation in sample clean-up. It also enables higher, more reproducible analytical recoveries of long-chain VFAs. The VFA content of the filtrate can also be measured by HPLC. Use of the ion-exclusion mechanism completely resolves isobutyric acid and butyric acid on a cation-exchange column. The mean (+/- SD) percentage distribution values of VFAs (measured by GLC) from five healthy subjects were 56.0 +/- 3.5 (acetic acid), 17.0 +/- 5.3 (propionic acid), 2.9 +/- 1.5 (isobutyric acid), 18.8 +/- 5.8 (butyric acid), 2.3 +/- 1.2 (isovaleric acid), and 2.9 +/- 0.8 (valeric acid).


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Nutr.Home page
J. Fernandes, A. V. Rao, and T. M. S. Wolever
Different Substrates and Methane Producing Status Affect Short-Chain Fatty Acid Profiles Produced by In Vitro Fermentation of Human Feces
J. Nutr., August 1, 2000; 130(8): 1932 - 1936.
[Abstract] [Full Text]


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Copyright © 1989 by the American Association for Clinical Chemistry.