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Clinical Chemistry 35: 86-89, 1989;
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Clinical Chemistry, Vol 35, 86-89, Copyright © 1989 by American Association for Clinical Chemistry

An immunoassay of human band 5 ("tartrate-resistant") acid phosphatase that involves the use of anti-porcine uteroferrin antibodies

KB Whitaker, TM Cox and DW Moss
Department of Chemical Pathology, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.

We describe an immunoassay for human band-5 acid phosphatase in which antibodies to porcine uteroferrin, immobilized on Sepharose particles, are used. Band-5 acid phosphatase is the tartrate-resistant isoenzyme normally expressed in tissue macrophages such as osteoclasts and alveolar macrophages. The immunoassay is similar in reproducibility and sensitivity to assays based on inhibition by d-tartrate. However, compared with the latter, the greater specificity of the immunoassay makes it markedly less susceptible to errors arising from the presence of non-band-5 acid phosphatases, e.g., from prostate.





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Copyright © 1989 by the American Association for Clinical Chemistry.