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Clinical Chemistry, Vol 35, 2098-2100, Copyright © 1989 by American Association for Clinical Chemistry
LC Allen, L Kadijevic and AD Romaschin
Department of Clinical Biochemistry, Toronto General Hospital, Ontario, Canada.
Measurement of oxalate in urine has been automated for use with the Cobas Fara centrifugal analyzer. No sample pretreatment other than (a critical) pH adjustment is required. Between-run CVs were less than 4%. Results were linearly related to oxalate concentration to 1000 mumol/L. Ascorbic acid ingestion, up to 5 g of ascorbate daily, caused no demonstrable interference with the assay. This practical, automated method for assaying urinary oxalate is substantially faster than other chemical or enzymatic methods.
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