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Clinical Chemistry 35: 2112-2115, 1989;
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Clinical Chemistry, Vol 35, 2112-2115, Copyright © 1989 by American Association for Clinical Chemistry

Fluorometric method for phenylalanine microplate assay adapted for phenylketonuria screening

NS Gerasimova, IV Steklova and T Tuuminen
All-Union Center for Phenylketonuria, Moscow, U.S.S.R.

We adapted the method of McCaman and Robins for fluorometry of phenylalanine to a microplate assay for routine phenylketonuria screening. Sensitivity is 15 mumol/L for the plasma assay and 30 mumol/L for the dried blood-spot assay, with CV less than 10% for both assays. Results for human plasma by microplate assay correlated well (r = 0.99) with results of amino acid analyzer determination of phenylalanine. When measurements are performed in an automated reader, the microplate assay has considerable advantages over conventional measurements in cuvettes: smaller volumes of reagents and automation enabling high throughput and convenience. Because the described method is a quantitative one, we can postulate that, compared with the semiquantitative Guthrie inhibition bioassay, this microplate assay is more reliable, easier to perform, and about twofold less costly.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
A. A. Reilly, R. Bellisario, and K. A. Pass
Multivariate discrimination for phenylketonuria (PKU) and non-PKU hyperphenylalaninemia after analysis of newborns' dried blood-spot specimens for six amino acids by ion-exchange chromatography
Clin. Chem., February 1, 1998; 44(2): 317 - 326.
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Copyright © 1989 by the American Association for Clinical Chemistry.