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Clinical Chemistry 35: 2116-2118, 1989;
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Clinical Chemistry, Vol 35, 2116-2118, Copyright © 1989 by American Association for Clinical Chemistry

Analysis for alpha-amylase isoenzymes by automated isoelectric focusing

F Lessard and R Dion
Service de biochimie, Hopital Saint-Luc, Montreal, Canada.

We describe the fractionation of alpha-amylase (EC 3.2.1.1) isoenzymes by use of the Pharmacia "Phastsystem" electrophoresis apparatus. The separation is done by isoelectric focusing on "Phastgel IEF 5-8." A complete run, including staining, takes 4 h and can easily resolve eight isoenzymes. Samples can be analyzed in a routine laboratory with use of conventional reagents.




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Copyright © 1989 by the American Association for Clinical Chemistry.