Clinical Chemistry
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Clinical Chemistry 35: 2124-2126, 1989;
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Clinical Chemistry, Vol 35, 2124-2126, Copyright © 1989 by American Association for Clinical Chemistry

Antipyrine, indocyanine green, and lorazepam determined in plasma by high-pressure liquid chromatography

WM Awni and LJ Bakker
Drug Evaluation Unit, Hennepin County Medical Center, Minneapolis, MN 55415.

This HPLC method for measuring antipyrine, lorazepam, and indocyanine green in 0.5 mL of plasma can be used in studies of liver function in which these "model" compounds are used. After a fast, simple, one-step extraction procedure with acetonitrile, an isocratic HPLC system is used, with a single detection wavelength (214 nm) and a single internal standard (1-acetamidopyrene). The mobile phase is a 47/53 (by vol) mixture of acetonitrile and 50 mmol/L phosphate buffer, pH 6. The three compounds are separated on an LC-18 reversed-phase column. The low cost of the HPLC method makes feasible the routine clinical measurement of all three compounds.


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Copyright © 1989 by the American Association for Clinical Chemistry.