Clinical Chemistry
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Clinical Chemistry 35: 2127-2129, 1989;
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Clinical Chemistry, Vol 35, 2127-2129, Copyright © 1989 by American Association for Clinical Chemistry

Total protein determined in human breast milk by use of coomassie brilliant blue and centrifugal analysis

Y Bergqvist, L Karlsson and L Fohlin
Department of Clinical Chemistry, Falun Central Hospital, Sweden.

This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.




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Copyright © 1989 by the American Association for Clinical Chemistry.