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Clinical Chemistry 35: 2219-2223, 1989;
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Clinical Chemistry, Vol 35, 2219-2223, Copyright © 1989 by American Association for Clinical Chemistry

Effect of glycation of low-density lipoprotein on the immunological determination of apolipoprotein B

RD Press and P Wilding
Department of Pathology, Hospital of the University of Pennsylvania, Philadelphia 19104.

Non-enzymatic glycation of low-density lipoprotein (LDL) may contribute to the premature atherogenesis of patients with diabetes mellitus. To assess whether glycation of apolipoprotein B, the predominant protein of LDL, interferes with the ability to immunologically quantify this protein, we prepared and purified glycated LDL by incubating normal plasma samples with high concentrations of glucose. Although both the plasma and the LDL specimens incubated with glucose contained significantly more glycated protein than control specimens, the quantitative interaction of an apolipoprotein B-specific antibody with glycated vs nonglycated LDL was not significantly different. We conclude that apolipoprotein B can be accurately quantified immunologically despite the presence of clinically excessive degrees of LDL glycation.




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Copyright © 1989 by the American Association for Clinical Chemistry.