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Clinical Chemistry 35: 2226-2227, 1989;
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Clinical Chemistry, Vol 35, 2226-2227, Copyright © 1989 by American Association for Clinical Chemistry

Estrogen and progesterone receptor assays, by the EIA method (Abbott), of low-speed supernates of breast-cancer homogenates

R Hahnel, MH Avenell and E Hahnel
Department of Obstetrics & Gynaecology, University of Western Australia, King Edward Memorial Hospital for Women, Subiaco.

Estrogen receptor (ER) and progesterone receptor (PR) assays were performed by solid-phase enzyme immunoassays (Abbott) on low-speed (2600 x g) and high-speed (100,000 x g) supernates of breast-cancer homogenates. The results were similar, indicating that ultracentrifugation is not essential for ER and PR assays with the Abbott kit.




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