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Clinical Chemistry 35: 2247-2249, 1989;
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Clinical Chemistry, Vol 35, 2247-2249, Copyright © 1989 by American Association for Clinical Chemistry

Simple enzymatic determination of total cholesterol in gallstones

JS Wei, HM Huang, WC Shyu and CS Wu
Department of Biochemistry, Chang Gung Medical College, Taoyuan, Taiwan, R.O.C.

In this simple method for rapid enzymatic determination of total cholesterol in human gallstones, gallstone powder is dissolved in an 80/20 by vol mixture of N,N-dimethylformamide and dimethyl sulfoxide and reacted directly with the aqueous enzymatic reagent, without further treatment. The organic solvents do not interfere with enzymatic activities of cholesterol oxidase, esterase, and peroxidase in the assay mixture. The method is reproducible (CVs 3.7% to 6.6%), analytical recoveries ranged from 98.6% to 102%, and linearity is good. Furthermore, results correlated well with those obtained by infrared spectroscopic measurements.




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Copyright © 1989 by the American Association for Clinical Chemistry.