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Clinical Chemistry 35: 2285-2289, 1989;
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Clinical Chemistry, Vol 35, 2285-2289, Copyright © 1989 by American Association for Clinical Chemistry

Simultaneous liquid-chromatographic determination of vitamin K1 and vitamin E in serum

BE Cham, HP Roeser and TW Kamst
Department of Medicine, University of Queensland, Royal Brisbane Hospital, Brisbane, Australia.

We describe a high-performance liquid chromatographic procedure for the simultaneous measurement of vitamins K1 and E in human serum. Delipidated human serum (free of vitamins K1 and E) was used to make standard solutions of these vitamins, and cetyl naphthoate and alpha- tocopheryl acetate were the internal standards for vitamin K1 and vitamin E, respectively. A simple, novel separation method utilizing liquid-liquid partition chromatography was used as a preparative "clean- up" procedure. Cetyl naphthoate and vitamin K1 (after post-column reduction) were detected by fluorescence, alpha-tocopheryl acetate and vitamin E by ultraviolet absorption. Sensitivity (detection limit) of the assay was 30 pg for vitamin K1 and 5 ng for vitamin E per injection. The method is specific, precise, and more rapid than previously described procedures. Within- and between-assay CVs were 8.1% and 12.9%, respectively, for vitamin K1; 3.5% and 6.0%, respectively, for vitamin E. Analytical recoveries of vitamins K1 and E were 80% and 93%, respectively, from serum and from delipidated serum (standards). The average neonatal serum concentration of vitamin K1 was 83 ng/L, 2.5 mg/L for vitamin E; for normolipidemic adults, the values were 343 ng/L and 7.9 mg/L, respectively, and for hyperlipidemic adults, 541 ng/L and 11.1 mg/L, respectively.


The following articles in journals at HighWire Press have cited this article:


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M. K. Azharuddin, D. St. J. O'Reilly, A. Gray, and D. Talwar
HPLC Method for Plasma Vitamin K1: Effect of Plasma Triglyceride and Acute-Phase Response on Circulating Concentrations
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B. E. Cham, J. L. Smith, and D. M. Colquhoun
What Happens to Vitamin K1 in Serum after Bone Fracture?
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B. E. Cham, J. L. Smith, and D. M. Colquhoun
Correlations between Cholesterol, Vitamin E, and Vitamin K1 in Serum: Paradoxical Relationships to Established Epidemiological Risk Factors for Cardiovascular Disease
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