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Clinical Chemistry 35: 2319-2321, 1989;
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Clinical Chemistry, Vol 35, 2319-2321, Copyright © 1989 by American Association for Clinical Chemistry

Chemiluminescent enzyme immunoassay of alpha-fetoprotein based on an adamantyl dioxetane phenyl phosphate substrate

GH Thorpe, I Bronstein, LJ Kricka, B Edwards and JC Voyta
Department of Clinical Chemistry, Wolfson Research Laboratories, Queen Elizabeth Medical Centre, Birmingham, U.K.

We have evaluated a new chemiluminescent substrate for the alkaline phosphatase (EC 3.1.3.1) label used in a Hybritech Tandem-E immunoassay of alpha-fetoprotein (AFP). The new substrate, adamantyl 1,2-dioxetane phenyl phosphate (AMPPD), emits light at 477 nm when acted upon by the enzyme. Detection limits for AFP with this method were 33 ng/L (mean of 20 replicates of the zero standard + 2 SD) and 470 ng/L (twice background). Between-batch CVs ranged from 4.31% to 9.60% for AFP in the range 29.1-132.0 micrograms/L. Comparison of results for 49 specimens assayed with use of the chemiluminescent kit and a colorimetric version of the AFP assay gave statistical values as follows: slope = 0.88, intercept = 4.19, and r = 0.94.


The following articles in journals at HighWire Press have cited this article:


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N. H.L. Chiu and T. K. Christopoulos
Two-Site Expression Immunoassay Using a Firefly Luciferase-coding DNA Label
Clin. Chem., November 1, 1999; 45(11): 1954 - 1959.
[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the American Association for Clinical Chemistry.