Clinical Chemistry
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Clinical Chemistry 35: 202-205, 1989;
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Clinical Chemistry, Vol 35, 202-205, Copyright © 1989 by American Association for Clinical Chemistry

Free 3-methoxy-4-hydroxyphenylglycol determined in plasma by liquid chromatography with coulometric detection

M Hariharan, T VanNoord, OG Cameron, GC Curtis and DG Ostrow
Department of Psychiatry, University of Michigan Medical School, Ann Arbor 48109-0656.

We describe a simple "high-performance" liquid-chromatographic method for determining free 3-methoxy-4-hydroxyphenylglycol (MHPG) in plasma, with coulometric detection and with 4-methoxy-3-hydroxyphenylglycol (iso-MHPG) as the internal standard. MHPG and iso-MHPG are extracted from plasma into ethyl acetate and the extract is washed with a sodium bicarbonate solution, evaporated, reconstituted, and injected into a 25 x 0.4 cm column packed with 3-micron particles of C18 material. We used a mobile phase of acetate buffer (100 mmol/L, pH 5.0) and methanol (92:8 by vol) and an oxidation voltage of 0.5 V. The detection limit of the assay is 0.1 micrograms/L. The interassay CV for a sample with a mean MHPG concentration of 3.84 micrograms/L was 5.2%. The average absolute recovery for the method was 37%.


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 Arch Gen PsychiatryHome page
J. L. Abelson, D. Glitz, O. G. Cameron, M. A. Lee, M. Bronzo, and G. C. Curtis
Blunted Growth Hormone Response to Clonidine in Patients With Generalized Anxiety Disorder
Arch Gen Psychiatry, February 1, 1991; 48(2): 157 - 162.
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Copyright © 1989 by the American Association for Clinical Chemistry.