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Clinical Chemistry 35: 321-322, 1989;
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Clinical Chemistry, Vol 35, 321-322, Copyright © 1989 by American Association for Clinical Chemistry

Homogentisic acid determined in biological fluids by HPLC

C Bory, R Boulieu, C Chantin and M Mathieu
Lab. de Biochimie, Hopital Debrousse, Lyon, France.

In this rapid, specific, and sensitive high-performance liquid- chromatographic method of analysis for homogentisic acid in biological fluids, homogentisic acid is separated on a column of Nucleosil CN. This method, which we applied to the diagnosis of three cases of alcaptonuria, represents a suitable analytical tool for the diagnosis of alcaptonuria.




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Copyright © 1989 by the American Association for Clinical Chemistry.