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Clinical Chemistry 35: 422-424, 1989;
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Clinical Chemistry, Vol 35, 422-424, Copyright © 1989 by American Association for Clinical Chemistry

Novel immunoradiometric assay of thyroglobulin in serum with use of monoclonal antibodies selected for lack of cross-reactivity with autoantibodies

M Piechaczyk, L Baldet, B Pau and JM Bastide
Unite de Recherche en Immunologie, Faculte de Pharmacie, Montpellier, France.

A multisite immunoradiometric assay for measurement of serum thyroglobulin (Tg), designated Magnogel-IRMA-Tg, has been developed, involving magnetic microbeads (Magnogel). This assay is based on the use of five anti-Tg monoclonal antibodies (MAbs) directed against three antigenic regions on the Tg molecule that are not recognized by anti-Tg autoantibodies (aAbs). Four of these MAbs, directed against two antigenic domains, were coupled to the magnetic beads and were used to trap the serum antigen. Another MAb, directed against the third region, was iodinated and served as the labeled second antibody. The Magnogel- IRMA-Tg technique is reproducible, rapid, and sensitive (lower detection limit, 3 micrograms/L). The assay reliably measures serum Tg in the presence of anti-Tg aAbs.


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Copyright © 1989 by the American Association for Clinical Chemistry.