Clinical Chemistry
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Clinical Chemistry 35: 456-458, 1989;
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Clinical Chemistry, Vol 35, 456-458, Copyright © 1989 by American Association for Clinical Chemistry

Screening for microalbuminuria by use of a rapid, low-cost colorimetric assay

G Phillipou, SK James, CJ Seaborn and PJ Phillips
Endocrine and Diabetes Laboratory, Queen Elizabeth Hospital, Woodville, South Australia.

We evaluated the pyrogallol red-molybdate(IV) method for quantification of urinary protein as a screening procedure for microalbuminuria by determining the assay's sensitivity and specificity at different concentrations of urinary albumin as measured by a comparison laser- nephelometric immunoassay. The pyrogallol red-molybdate(IV) method has sensitivity and specificity similar to that for other semiquantitative assays, but it is less expensive and sample throughput can be high if microtiter plate techniques are used.


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 Clin. Chem.Home page
T. Marshall and K. M. Williams
Total Protein Determination in Urine: Elimination of a Differential Response between the Coomassie Blue and Pyrogallol Red Protein Dye-binding Assays
Clin. Chem., March 1, 2000; 46(3): 392 - 398.
[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the American Association for Clinical Chemistry.