Clinical Chemistry
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Clinical Chemistry 35: 478-481, 1989;
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Clinical Chemistry, Vol 35, 478-481, Copyright © 1989 by American Association for Clinical Chemistry

Monoclonal immunoglobulins: affinity blotting for low concentrations in serum

R McLachlan
Pathology Department, Peter MacCallum Cancer Institute, Melbourne, Australia.

I describe a simple, economical technique for identifying low concentrations of monoclonal immunoglobulins in the presence of excessive amounts of immunoglobulins of other classes. The technique involves binding of specific antibody to nitrocellulose, separating proteins by isoelectric focusing or zone electrophoresis in agarose gels, using capillary transfer to bind proteins to the nitrocellulose via their antibody affinity, and then detecting transferred proteins with enzyme-labeled antibody. A monoclonal immunoglobulin can be completely characterized in 2 h. No expensive equipment is required. Affinity blotting is about 10-fold as sensitive as native blotting, 100- fold as sensitive as silver staining.




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Copyright © 1989 by the American Association for Clinical Chemistry.