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Clinical Chemistry 35: 552-554, 1989;
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Clinical Chemistry, Vol 35, 552-554, Copyright © 1989 by American Association for Clinical Chemistry

Sensitive, direct colorimetric assay for copper in serum

A Abe, S Yamashita and A Noma
Department of Laboratory Medicine, Gifu University School of Medicine, Japan.

We have developed a sensitive procedure for determination of serum copper by use of the color reagent 4-(3,5-dibromo-2-pyridylazo)-N-ethyl- N-sulfopropylaniline. After mixing serum sample and reagent, and incubating at 37 degrees C for 5 min, we measure the absorbance of the resulting chelate complex at 580 nm (molar absorptivity, 80,000 L.mol- 1.cm-1). Results of the method varied linearly with copper concentration to at least 5 mg/L; the lower limit of detection was 0.1 mg/L. Within-run CVs were 1.6% and 3.3% for copper concentrations of 1.03 and 0.72 mg/L, respectively (n = 10 each). Between-run CV was 2.8% at 1.22 mg/L (n = 14). Results of the proposed method (y) correlated well with those determined by standard atomic absorption spectrophotometric techniques (x): y = 0.99x - 0.02 mg/L; Syx = 0.08; r = 0.977; n = 56. Iron, zinc, cadmium, cobalt, and lead do not interfere.


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Copyright © 1989 by the American Association for Clinical Chemistry.