Clinical Chemistry
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Clinical Chemistry 35: 569-572, 1989;
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Clinical Chemistry, Vol 35, 569-572, Copyright © 1989 by American Association for Clinical Chemistry

Solid-phase enzymoimmunoassay of estrone in saliva

J Folan, JP Gosling, MF Finn and PF Fottrell
Department of Biochemistry, University College, Galway, Ireland.

In this solid-phase enzymoimmunoassay for estrone in saliva, microtiter plates are used after extraction of the sample with diethyl ether. No chromatographic step is involved. The detection limit of the assay is 450 fg per well (33 pmol/L). Intra- and interassay coefficients of variation for the assay of low, medium, and high concentrations of estrone in saliva were respectively 4.2, 12.7; 5.2, 8.7; and 2.7, 5.8%. Using this assay, we found a highly significant correlation (P less than 0.001) between estrone concentrations in time-matched serum and saliva samples. The analytical procedure is rapid and relatively simple. One person can assay 50-60 saliva samples during a normal working day. We conclude that the assay is very suitable, even in small laboratories, for saliva estrone measurements, which, in facilitating serial sampling, enables dynamic observations of estrone concentrations and ovarian activity to be more easily made.




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Copyright © 1989 by the American Association for Clinical Chemistry.