Clinical Chemistry
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Clinical Chemistry 35: 766-772, 1989;
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Clinical Chemistry, Vol 35, 766-772, Copyright © 1989 by American Association for Clinical Chemistry

Simultaneous measurement of total and IgA-conjugated alpha 1- microglobulin by a combined immunoenzyme/immunoradiometric assay technique

DD DeMars, JA Katzmann, TK Kimlinger, JD Calore and RP Tracy
Department of Pathology, University of Vermont, Burlington 05405.

alpha 1-Microglobulin (alpha 1m), a glycoprotein (Mr = 30,000) found in serum and urine, is also present in serum conjugated to monomeric IgA (alpha 1-m-IgA). We have developed a simultaneous enzyme-linked immunoenzyme/immunoradiometric assay that involves three different monoclonal antibodies. Assay of serial dilutions of serum and urine demonstrated parallelism. Normal mean concentrations in serum (n = 75) were: total alpha 1m, 2.33 mumol/L; alpha 1m-IgA, 1.24 mumol/L; unconjugated (free) alpha 1m, 1.09 mumol/L; molar ratio (alpha 1m- IgA/total alpha 1m), 0.53. The mean concentration of alpha 1m in eight urine specimens from normal individuals was 0.19 mumol/L, with no detectable alpha 1m-IgA. A low urinary pH does not significantly affect assay results, unlike assays of beta 2-microglobulin. In patients with myeloma-related renal disease, total and free alpha 1m values for serum correlated well with values for creatinine and beta 2-microglobulin in serum.


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B. Akerstrom, G. J. Maghzal, C. C. Winterbourn, and A. J. Kettle
The Lipocalin {alpha}1-Microglobulin Has Radical Scavenging Activity
J. Biol. Chem., October 26, 2007; 282(43): 31493 - 31503.
[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the American Association for Clinical Chemistry.