Clinical Chemistry
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Clinical Chemistry 35: 821-823, 1989;
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Clinical Chemistry, Vol 35, 821-823, Copyright © 1989 by American Association for Clinical Chemistry

Determination of the alkyl lysophospholipid derivative ET-18-OCH3, a new antineoplastic drug, in plasma

HG Schaefer and P Rohdewald
Institut fur Pharmazeutische Chemie, Westfalische Wilhelms-Universitat, Munster, F.R.G.

We describe a sensitive method for measuring the concentration of the new antineoplastic drug ET-18-OCH3 in plasma. After plasma lipids are extracted, ET-18-OCH3 is separated from the excess of endogenous lipids by thin-layer chromatography and specific enzymatic hydrolysis of sphingomyelin by the action of sphingomyelinase. Analytical recovery after the complete isolation was 73.5% (CV = 9.8%, n = 15). [3H]-ET-18- OCH3 is used as internal standard. A densitometric method in which 8- anilino-1-naphthalenesulfonate, Mg salt, is used as fluorescent agent (excitation at 367 nm and emission greater than 390 nm) allows the sensitive determination of ET-18-OCH3 down to 0.1 mg/L (CV greater than 30%). The day-to-day CV is 25% for concentrations of 0.15 to 0.625 mg/L, 12% for 1.5 to 5.0 mg/L. Preliminary pharmacokinetic data reveal gastrointestinal absorption of ET-18-OCH3 after multiple oral administration.




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Copyright © 1989 by the American Association for Clinical Chemistry.