Clinical Chemistry
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Clinical Chemistry 35: 831-833, 1989;
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Clinical Chemistry, Vol 35, 831-833, Copyright © 1989 by American Association for Clinical Chemistry

An automated procedure for measuring biotinidase activity in serum

KA Weissbecker, HD Gruemer, GS Heard, WG Miller, WE Nance and B Wolf
Department of Human Genetics, Medical College of Virginia of Virginia Commonwealth University, Richmond 23298-0033.

In this automated procedure for quantifying biotinidase activity in human serum, a manual colorimetric method that measures conversion of the enzyme's artificial substrate N-biotinyl p-aminobenzoate was modified for use with a Technicon AutoAnalyzer II. The intra-run replicate precision (CV) was 2.1% and the day-to-day CV was 4.6% for quality-control sera. Results were linearly related to biotinidase activity in serum over the complete range of clinically relevant values, 0.2 to 11.0 U/L. Moreover, results of the automated assay were not significantly different from those of the manual assay. Because the automated procedure is faster and more precise, we recommend it for population-based studies and some screening studies.




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Copyright © 1989 by the American Association for Clinical Chemistry.