Clinical Chemistry
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Clinical Chemistry 35: 835-837, 1989;
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Clinical Chemistry, Vol 35, 835-837, Copyright © 1989 by American Association for Clinical Chemistry

A new commercial method for the enzymatic determination of creatinine in serum and urine evaluated: comparison with a kinetic Jaffe method and isotope dilution-mass spectrometry

B Lindback and A Bergman
Department of Clinical Chemistry, Huddinge University Hospital, Sweden.

We evaluated a new, simple, enzymatic kinetic method from Wako Chemicals GmbH in comparison with a kinetic Jaffe method by using isotope dilution-mass spectrometry (ID-MS) as a reference method. An ID- MS-calibrated serum standard was used. Both the enzymatic and the Jaffe method correlated well with ID-MS, except for sera with high concentrations of bilirubin. Ethyl acetoacetate, acetone, and glucose in serum interfered somewhat with the Jaffe method but not with the enzymatic method. We conclude that the present enzymatic method has merit as compared with a Jaffe method for routine work, but is more expensive.


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Copyright © 1989 by the American Association for Clinical Chemistry.