Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 35: 884-887, 1989;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by D'Haese, P. C.
Right arrow Articles by De Broe, M. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by D'Haese, P. C.
Right arrow Articles by De Broe, M. E.

Clinical Chemistry, Vol 35, 884-887, Copyright © 1989 by American Association for Clinical Chemistry

Indirect measurement of desferrioxamine and its chelated compounds aluminoxamine and ferrioxamine by Zeeman atomic absorption spectrometry

PC D'Haese, LV Lamberts and ME De Broe
Department of Nephrology-Hypertension, University of Antwerp, Belgium.

This indirect method for determination of desferrioxamine (DFO, deferoxamine, Desferal) and its chelated compounds ferrioxamine (FO) and aluminoxamine (AIO) in biological fluids involves metal chelate formation and solvent extraction, the metal being determined by Zeeman atomic absorption spectrometry (Zeeman-AAS). Special attention was paid to the pH and recovery of extraction, iron-aluminum interactions, extraction times and volumes, centrifugation rates, and instrument settings. Precautions were taken to avoid contamination with aluminum and iron and to prevent co-extraction of iron and aluminum not chelated to DFO. The addition calibration technique was used for standardization. Mean +/- SD recoveries of AIO and FO after extraction were respectively 98.3% +/- 3.7% and 101% +/- 3.7%. Sensitivity was 45 pg/0.0044 A.s for aluminum and 10 pg/0.0044 A.s for iron. Detection limits were 0.22 mumol/L for AIO, 0.06 mumol/L for DFO and FO.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
P. Menendez-Fraga, J. L. Fernandez-Martin, E. Blanco-Gonzalez, and J. B. Cannata-Andia
Low percentage of aluminoxamine and ferrioxamine in uremic serum after desferrioxamine administration
Clin. Chem., June 1, 1998; 44(6): 1262 - 1268.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1989 by the American Association for Clinical Chemistry.