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Clinical Chemistry 35: 1000-1004, 1989;
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Clinical Chemistry, Vol 35, 1000-1004, Copyright © 1989 by American Association for Clinical Chemistry

Enzyme-linked immunosorbent assay of apolipoprotein B in blood spotted onto filter paper, suitable for neonatal screening

XL Wang, NP Dudman and DE Wilcken
Department of Cardiovascular Medicine, Prince Henry Hospital, University of New South Wales, Sydney, N.S.W., Australia.

This assay was developed to measure apolipoprotein (apo) B in blood samples spotted onto filter paper. The long-term aim is to detect young families with the dominantly inherited familial hypercholesterolemia, during current neonatal screening programs. The interassay CV was 5.6%, and apo B concentrations correlated closely with values measured by radial immunodiffusion. In the present assay, the primary apo B standard selected and the serum samples behaved similarly. Use of Triton X-100 in the extraction of apo B from dried blood prevented the decrease in apo B immunoreactivity that otherwise occurred during storage for 20 days at 4 degrees C. In 57 neonates two to six days postpartum, the mean (and SD) apo B concentration in whole blood was 186 (78) mg/L, and the apo A-I/apo B ratio was higher in female than in male neonates (P less than 0.001), as is also true for adults. The assay is suitable for use in screening programs for newborns, and the observations add to our understanding of lipid metabolism in neonates.


The following articles in journals at HighWire Press have cited this article:


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 Arterioscler. Thromb. Vasc. Bio.Home page
A. F. Vuorio, H. Turtola, and K. Kontula
Neonatal Diagnosis of Familial Hypercholesterolemia in Newborns Born to a Parent With a Molecularly Defined Heterozygous Familial Hypercholesterolemia
Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 3332 - 3337.
[Abstract] [Full Text]

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 CirculationHome page
X.L. Wang, R.M. McCredie, and D.E.L. Wilcken
Common DNA Polymorphisms at the Lipoprotein Lipase Gene : Association With Severity of Coronary Artery Disease and Diabetes
Circulation, April 1, 1996; 93(7): 1339 - 1345.
[Abstract] [Full Text]


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