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Clinical Chemistry 35: 1043-1044, 1989;
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Clinical Chemistry, Vol 35, 1043-1044, Copyright © 1989 by American Association for Clinical Chemistry

Thin-layer chromatography of free porphyrins for diagnosis of porphyria

MJ Henderson
Department of Chemical Pathology, St. James's University Hospital, Leeds, U.K.

In this simple thin-layer chromatographic (TLC) technique for evaluating porphyrin excretion, porphyrins are extracted from urine or feces, then separated on silica-gel TLC plates. The distinct porphyrin bands are observed by viewing the plates under long-wave fluorescent light. Positive screening tests can readily be confirmed or rejected, and a more comprehensive investigation confidently undertaken.




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Copyright © 1989 by the American Association for Clinical Chemistry.