Clinical Chemistry
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Clinical Chemistry 35: 1043-1044, 1989;
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Clinical Chemistry, Vol 35, 1043-1044, Copyright © 1989 by American Association for Clinical Chemistry

Thin-layer chromatography of free porphyrins for diagnosis of porphyria

MJ Henderson
Department of Chemical Pathology, St. James's University Hospital, Leeds, U.K.

In this simple thin-layer chromatographic (TLC) technique for evaluating porphyrin excretion, porphyrins are extracted from urine or feces, then separated on silica-gel TLC plates. The distinct porphyrin bands are observed by viewing the plates under long-wave fluorescent light. Positive screening tests can readily be confirmed or rejected, and a more comprehensive investigation confidently undertaken.


The following articles in journals at HighWire Press have cited this article:


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D.-L. Lin, L.-F. He, and Y.-Q. Li
Rapid and Simultaneous Determination of Coproporphyrin and Protoporphyrin in Feces by Derivative Matrix Isopotential Synchronous Fluorescence Spectrometry
Clin. Chem., October 1, 2004; 50(10): 1797 - 1803.
[Abstract] [Full Text] [PDF]

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M. K. Akhtar, N. N. Kaderbhai, D. J. Hopper, S. L. Kelly, and M. A. Kaderbhai
Export of a Heterologous Cytochrome P450 (CYP105D1) in Escherichia coli Is Associated with Periplasmic Accumulation of Uroporphyrin
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[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the American Association for Clinical Chemistry.