Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 35: 1288-1292, 1989;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kirk, E. M.
Right arrow Articles by Fell, A. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kirk, E. M.
Right arrow Articles by Fell, A. F.

Clinical Chemistry, Vol 35, 1288-1292, Copyright © 1989 by American Association for Clinical Chemistry

Analysis of supplemented vitamin K1(20) in serum microsamples by solid- phase extraction and narrow-bone HPLC with multichannel ultraviolet detection

EM Kirk and AF Fell
Pharmaceutical Chemistry, School of Pharmacy, University of Bradford, U.K.

A sensitive method for the determination of vitamin K1(20) in serum microsamples (50 microL) has been developed, utilizing solid-phase extraction with C8 Bond-Elut columns and reversed-phase narrow-bore high-performance liquid chromatography [2.1 mm (i.d.), column] with a nonaqueous eluent. Recovery from serum (49 ng/mL) was 76% (n = 2). Peak homogeneity was assessed by photodiode array detection with absorbance ratio, spectral normalization, and transformation to the first- and second-derivative chromatograms. Calibration data at 248 nm over two ranges (20-200 ng/mL, 200-4000 ng/mL) varied linearly with concentration and were suitable for studies of vitamin K1 supplementation. By comparison with conventional columns, sensitivity was increased twofold.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1989 by the American Association for Clinical Chemistry.