Clinical Chemistry
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Clinical Chemistry 35: 1404-1407, 1989;
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Clinical Chemistry, Vol 35, 1404-1407, Copyright © 1989 by American Association for Clinical Chemistry

Determination of magnesium in granulocytes

JM Hosseini, XY Yang and RJ Elin
Clinical Pathology Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892.

Determination of magnesium in mononuclear blood cells may provide useful information about intracellular magnesium. Mononuclear blood cell preparations are frequently contaminated with granulocytes. We determined the normal reference interval for magnesium content and concentration of granulocytes and compared it with magnesium in mononuclear blood cells. The mean granulocyte magnesium concentration and content were 6.95 (SD 1.25) mmol/L and 3.11 (SD 0.54) fmol per cell, respectively, vs 10.95 (SD 1.70) mmol/L and 3.27 (SD 0.46) fmol per cell in mononuclear cells. The concentration of magnesium in granulocytes was significantly less (P less than 0.001) than in mononuclear blood cells, but the magnesium contents were not significantly different.




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Copyright © 1989 by the American Association for Clinical Chemistry.