Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 35: 1452-1455, 1989;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Puleo, P. R.
Right arrow Articles by Perryman, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Puleo, P. R.
Right arrow Articles by Perryman, M. B.

Clinical Chemistry, Vol 35, 1452-1455, Copyright © 1989 by American Association for Clinical Chemistry

Sensitive, rapid assay of subforms of creatine kinase MB in plasma

PR Puleo, PA Guadagno, R Roberts and MB Perryman
Department of Medicine, Baylor College of Medicine, Houston, TX 77030.

The subforms of creatine kinase (CK; EC 2.7.3.2) in plasma have received recent attention as potential markers for the early diagnosis of acute myocardial infarction. Because changes in CK-MM subforms are not specific for myocardial injury, we developed an assay, based on high-voltage electrophoresis, that is sufficiently sensitive to detect the CK-MB subforms at concentrations substantially below the upper limit of normal (14 U/L). The assay can detect 1.25 U of either MB subform per liter with a precision of 0.20 U/L and gives responses that vary linearly with activity concentration from 0.0 through 30.0 U/L, with an identical signal response for both subforms. When both subforms are present in a serum sample, the assay accurately measures both the relative percentage and the absolute quantity of each: assay activity/known activity was 1.03 for each subform at a total MB subform activity of 5.0 U/L (r = 0.98). Assay time is 25 min, and there is no loss of CK during electrophoresis. Thus, this system can be used to rapidly, sensitively, and precisely quantify the two CK-MB subforms at activities well within the normal reference interval.


The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Clin. Nutr.Home page
K.-A. da Costa, M. Badea, L. M Fischer, and S. H Zeisel
Elevated serum creatine phosphokinase in choline-deficient humans: mechanistic studies in C2C12 mouse myoblasts
Am. J. Clinical Nutrition, July 1, 2004; 80(1): 163 - 170.
[Abstract] [Full Text] [PDF]

Home page
 Br J AnaesthHome page
M. Kemp, J. Donovan, H. Higham, and J. Hooper
Biochemical markers of myocardial injury
Br. J. Anaesth., July 1, 2004; 93(1): 63 - 73.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
R. Edwards, T. Treasure, M. Hossein-Nia, A. Murday, G. H. Kantidakis, and D. W. Holt
A controlled trial of substrate-enhanced, warm reperfusion (""hot shot"") versus simple reperfusion
Ann. Thorac. Surg., February 1, 2000; 69(2): 551 - 555.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. H.B. Wu, F. S. Apple, W. B. Gibler, R. L. Jesse, M. M. Warshaw, and R. Valdes Jr.
National Academy of Clinical Biochemistry Standards of Laboratory Practice: Recommendations for the Use of Cardiac Markers in Coronary Artery Diseases
Clin. Chem., July 1, 1999; 45(7): 1104 - 1121.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
I. Birdi, G. D. Angelini, and A. J. Bryan
Biochemical Markers of Myocardial Injury During Cardiac Operations
Ann. Thorac. Surg., March 1, 1997; 63(3): 879 - 884.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
E. W. Bush, C. S. Taft, G. E. Meixell, and M. B. Perryman
Overexpression of Myotonic Dystrophy Kinase in BC(3)H1 Cells Induces the Skeletal Muscle Phenotype
J. Biol. Chem., January 5, 1996; 271(1): 548 - 552.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
P. R. Puleo, D. Meyer, C. Wathen, C. B. Tawa, S. Wheeler, R. J. Hamburg, N. Ali, S. D. Obermueller, F. J. Triana, J. L. Zimmerman, et al.
Use of a Rapid Assay of Subforms of Creatine Kinase MB to Diagnose or Rule Out Acute Myocardial Infarction
N. Engl. J. Med., September 1, 1994; 331(9): 561 - 566.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1989 by the American Association for Clinical Chemistry.