Clinical Chemistry
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Clinical Chemistry 35: 1467-1471, 1989;
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Clinical Chemistry, Vol 35, 1467-1471, Copyright © 1989 by American Association for Clinical Chemistry

Neopterin measured in serum and tissue culture supernates by a competitive enzyme-linked immunosorbant assay

M Barak, D Merzbach and N Gruener
Department of Biochemistry, Carmel Hospital, Haifa, Israel.

In this solid-phase competitive enzyme-linked immunosorbant assay for neopterin (a product of activated macrophages) in serum or supernates of cell cultures, we incubate antiserum to neopterin with standards or samples in the presence of solid-phase-bound conjugate of carrier- neopterin. Incubation with second antibody labeled with peroxidase then follows, before reaction with substrate. Analytical recovery, precision, and sensitivity of this method are similar to those of other immunoassays. The assay range is between 0.4 and 100 micrograms of neopterin per liter. Results obtained by this method compared with those from a conventional radioimmunoassay gave a correlation coefficient of 0.974.




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Copyright © 1989 by the American Association for Clinical Chemistry.