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Clinical Chemistry, Vol 35, 1609-1614, Copyright © 1989 by American Association for Clinical Chemistry
H Dechaud, H Lejeune, M Garoscio-Cholet, R Mallein and M Pugeat
Laboratoire Central de Biochimie, Hopital de l'Antiquaille, Hospices Civils de Lyon, France.
To measure the concentration of testosterone (T) that is not bound to sex-steroid-binding protein (SBP) in plasma, we quantified by radioimmunoassay the T in the supernates of plasma samples after precipitation with 50%-saturated ammonium sulfate. The concentrations of non-SBP-bound T. directly measured with this assay, correlated significantly (P less than 0.001) with those deduced from measurement of the percentage of non-SBP-bound T determined with [3H]T as tracer or from mathematical models according to the law of mass action. It also correlated significantly with the ratio of T to SBP and with the concentration of nonbound T. As determined with this assay, the mean concentration of non-SBP-bound T in normal men was higher in young (4.67, SD 2.68 nmol/L; n = 30) than in older (greater than 40 years) subjects (2.48, SD 1.61 nmol/L; n = 35; P less than 0.001) and lower than normal in hyperthyroid (1.61, SD 0.91 nmol/L; P less than 0.01) or infertile men (3.28, SD 1.70 nmol/L; P less than 0.01). In women, non- SBP-bound T was higher in hirsute patients (0.24, SD 0.11 nmol/L; P less than 0.01) and was lower during pregnancy (0.09, SD 0.05 nmol/L; P less than 0.05) than in normal women during the follicular phase (0.16, SD 0.07 nmol/L). We conclude that this direct measurement of non-SBP- bound T in plasma is suitable for routine use and represents a reliable index of androgenicity in human pathology, particularly when alterations of the binding capacity of SBP modify the concentrations of total T.
The following articles in journals at HighWire Press have cited this article:
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  V. Tronchon, F. Vialard, M. El Sirkasi, H. Dechaud, J. Rollet, M. Albert, M. Bailly, P. Roy, C. Mauduit, P. Fenichel, et al. Tumor necrosis factor-alpha -308 polymorphism in infertile men with altered sperm production or motility Hum. Reprod., December 1, 2008; 23(12): 2858 - 2866. [Abstract] [Full Text] [PDF]
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 H. Dechaud, A. Denuziere, S. Rinaldi, J. Bocquet, H. Lejeune, and M. Pugeat Age-Associated Discrepancy between Measured and Calculated Bioavailable Testosterone in Men Clin. Chem., April 1, 2007; 53(4): 723 - 728. [Abstract] [Full Text] [PDF]
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 O. Levillain, G. Ventura, H. Dechaud, M. Hobeika, A. Meseguer, C. Moinard, and L. Cynober Sex-differential expression of ornithine aminotransferase in the mouse kidney Am J Physiol Renal Physiol, March 1, 2007; 292(3): F1016 - F1027. [Abstract] [Full Text] [PDF]
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 N. Nader, G. Raverot, A. Emptoz-Bonneton, H. Dechaud, M. Bonnay, E. Baudin, and M. Pugeat Mitotane Has an Estrogenic Effect on Sex Hormone-Binding Globulin and Corticosteroid-Binding Globulin in Humans J. Clin. Endocrinol. Metab., June 1, 2006; 91(6): 2165 - 2170. [Abstract] [Full Text] [PDF]
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F. Giton, J. Fiet, J. Guechot, F. Ibrahim, F. Bronsard, D. Chopin, and J.-P. Raynaud Serum Bioavailable Testosterone: Assayed or Calculated? Clin. Chem., March 1, 2006; 52(3): 474 - 481. [Abstract] [Full Text] [PDF]
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 O. Levillain, J.-J. Diaz, O. Blanchard, and H. Dechaud Testosterone Down-Regulates Ornithine Aminotransferase Gene and Up-Regulates Arginase II and Ornithine Decarboxylase Genes for Polyamines Synthesis in the Murine Kidney Endocrinology, February 1, 2005; 146(2): 950 - 959. [Abstract] [Full Text] [PDF]
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P. Cousin, L. Calemard-Michel, H. Lejeune, G. Raverot, N. Yessaad, A. Emptoz-Bonneton, Y. Morel, and M. Pugeat Influence of SHBG Gene Pentanucleotide TAAAA Repeat and D327N Polymorphism on Serum Sex Hormone-Binding Globulin Concentration in Hirsute Women J. Clin. Endocrinol. Metab., February 1, 2004; 89(2): 917 - 924. [Abstract] [Full Text] [PDF]
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P.-H. Ducluzeau, P. Cousin, E. Malvoisin, H. Bornet, H. Vidal, M. Laville, and M. Pugeat Glucose-to-Insulin Ratio Rather than Sex Hormone-Binding Globulin and Adiponectin Levels Is the Best Predictor of Insulin Resistance in Nonobese Women with Polycystic Ovary Syndrome J. Clin. Endocrinol. Metab., August 1, 2003; 88(8): 3626 - 3631. [Abstract] [Full Text] [PDF]
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 S. Rinaldi, A. Geay, H. Dechaud, C. Biessy, A. Zeleniuch-Jacquotte, A. Akhmedkhanov, R. E. Shore, E. Riboli, P. Toniolo, and R. Kaaks Validity of Free Testosterone and Free Estradiol Determinations in Serum Samples from Postmenopausal Women by Theoretical Calculations Cancer Epidemiol. Biomarkers Prev., October 1, 2002; 11(10): 1065 - 1071. [Abstract] [Full Text] [PDF]
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R. Davies, C. Collier, M. Raymond, J. Heaton, and A. Clark Indirect Measurement of Bioavailable Testosterone with the Bayer Immuno 1 System Clin. Chem., February 1, 2002; 48(2): 388 - 390. [Full Text] [PDF]
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