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Clinical Chemistry 35: 1644-1648, 1989;
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Clinical Chemistry, Vol 35, 1644-1648, Copyright © 1989 by American Association for Clinical Chemistry

Functional activity of protein S determined with use of protein C activated by venom activator

I Kobayashi, N Amemiya, T Endo, K Okuyama, K Tamura and S Kume
2nd Department of Internal Medicine, Yamanashi Medical College, Japan.

A new screening procedure, an easy and specific assay for determining functional Protein S activity, has been developed, with use of Protein C activated by venom activator (Protac). Purified Protein C (100% amidolytic activity) was activated by venom activator (6 units/mL). To a mixture of 100 microL of Protein S-deficient plasma, 20 microL of sample plasma, 100 microL of cephalin (Actin), and 20 microL of activated Protein C we added 100 microL of 25 mmol/L CaCl2 solution and measured the clotting time with a KC 10 coagulometer. The functional Protein S activity correlated well with the concentrations of Protein S antigen measured by enzyme immunoassay and the Laurell rocket technique (r = 0.810 and 0.850, respectively) in normal subjects, patients with myocardial infarction undergoing warfarin therapy, and patients with liver cirrhosis.


The following articles in journals at HighWire Press have cited this article:


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S. Hirokawa and E. F. Mammen
A Functional Protein S and Microlatex Immunoassay for Protein S and C4b-Binding Protein on the Automated Coagulation Laboratory (ACL) 300 Plus
Clinical and Applied Thrombosis/Hemostasis, October 1, 1996; 2(4): 268 - 275.
[Abstract] [PDF]


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Copyright © 1989 by the American Association for Clinical Chemistry.