Clinical Chemistry
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Clinical Chemistry 35: 1644-1648, 1989;
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Clinical Chemistry, Vol 35, 1644-1648, Copyright © 1989 by American Association for Clinical Chemistry

Functional activity of protein S determined with use of protein C activated by venom activator

I Kobayashi, N Amemiya, T Endo, K Okuyama, K Tamura and S Kume
2nd Department of Internal Medicine, Yamanashi Medical College, Japan.

A new screening procedure, an easy and specific assay for determining functional Protein S activity, has been developed, with use of Protein C activated by venom activator (Protac). Purified Protein C (100% amidolytic activity) was activated by venom activator (6 units/mL). To a mixture of 100 microL of Protein S-deficient plasma, 20 microL of sample plasma, 100 microL of cephalin (Actin), and 20 microL of activated Protein C we added 100 microL of 25 mmol/L CaCl2 solution and measured the clotting time with a KC 10 coagulometer. The functional Protein S activity correlated well with the concentrations of Protein S antigen measured by enzyme immunoassay and the Laurell rocket technique (r = 0.810 and 0.850, respectively) in normal subjects, patients with myocardial infarction undergoing warfarin therapy, and patients with liver cirrhosis.


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S. Hirokawa and E. F. Mammen
A Functional Protein S and Microlatex Immunoassay for Protein S and C4b-Binding Protein on the Automated Coagulation Laboratory (ACL) 300 Plus
Clinical and Applied Thrombosis/Hemostasis, October 1, 1996; 2(4): 268 - 275.
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Copyright © 1989 by the American Association for Clinical Chemistry.