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Clinical Chemistry 35: 1706-1712, 1989;
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Clinical Chemistry, Vol 35, 1706-1712, Copyright © 1989 by American Association for Clinical Chemistry

A 125I-radioimmunoassay for measuring androstenedione in serum and in blood-spot samples from neonates

S Thomson, AM Wallace and B Cook
Department of Bacteriology, Stobhill Hospital, Glasgow, U.K.

We developed a radioimmunoassay with a gamma-emitting radioligand to measure androstenedione in human serum and in dried blood-spot samples from newborns. Antisera were raised in rabbits against androstenedione linked to bovine serum albumin at positions 3, 6, or 11 on the steroid nucleus. Radioligands were prepared by linking [125I]iodohistamine at positions 3, 6, or 11. Linkages were through either carboxymethyloxime or hemisuccinate bridges. All label and antibody combinations were examined, and the most sensitive and specific combination (antiserum raised against androstenedione-3-carboxymethyloxime-bovine serum albumin with an androstenedione-carboxymethyloxime-[125I]iodohistamine label) was selected for full evaluation. We report the performance of these selected reagents in an immunoassay for androstenedione in both serum and dried blood-spot samples from neonates. We measured concentrations of androstenedione in serum under normal and pathological conditions such as congenital adrenal hyperplasia and polycystic ovarian disease. Diurnal variation in normal men was observed. Androstenedione was measured in blood spots from neonates born at term or prematurely, with respiratory distress syndrome, or with congenital adrenal hyperplasia.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Clin. Endocrinol. Metab.Home page
A. Jamieson, A. M. Wallace, R. Andrew, B. S. Nunez, B. R. Walker, R. Fraser, P. C. White, and J. M. C. Connell
Apparent Cortisone Reductase Deficiency: A Functional Defect in 11{beta}-Hydroxysteroid Dehydrogenase Type 1
J. Clin. Endocrinol. Metab., October 1, 1999; 84(10): 3570 - 3574.
[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the American Association for Clinical Chemistry.