Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 35: 1721-1722, 1989;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hansen, S. I.
Right arrow Articles by Holm, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hansen, S. I.
Right arrow Articles by Holm, J.

Clinical Chemistry, Vol 35, 1721-1722, Copyright © 1989 by American Association for Clinical Chemistry

Quantification of biotin in serum by competition with solid-phase biotin for binding to peroxidase-avidin conjugate

SI Hansen and J Holm
Department of Clinical Chemistry, Central Hospital Hillerod, Denmark.

We have developed a simple, fast, accurate, and reproducible method--an Enzyme-Linked Ligand Sorbent Assay (ELLSA)--for quantifying biotin without the use of isotopes and immunological reagents. The analytical principle is based on the competition between biotin of the sample and immobilized biotin, i.e., biotinylated bovine serum albumin coated onto immunoplates, for binding to horseradish peroxidase (EC 1.11.1.7)- avidin conjugate. The amount of conjugate bound to immobilized biotin is subsequently measured.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1989 by the American Association for Clinical Chemistry.