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Clinical Chemistry 35: 1726-1730, 1989;
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Clinical Chemistry, Vol 35, 1726-1730, Copyright © 1989 by American Association for Clinical Chemistry

Measurement of cyclosporine in plasma from patients with various transplants: HPLC radioimmunoassay with a specific monoclonal antibody compared

JH McBride, DO Rodgerson, SS Park and AF Reyes
Department of Pathology, U.C.L.A. School of Medicine 90024-1732.

This study compares cyclosporin A (CsA) concentrations in plasma from patients receiving various transplants, as measured by HPLC and RIA with a monoclonal antibody for CsA and an 125I-labeled ligand. The RIA was restandardized with in-house standards because it overestimated CsA by an average of 23%. The RIA was sensitive to 2 micrograms/L, the standard curve was linear from 20 to 500 micrograms of CsA per liter, analytical recovery was 98%, and CVs were less than 8% for intra- and interassay precision. RIA (y) vs HPLC (x) for 283 plasma samples from 145 patients gave a slope = 1.1256, r = 0.979. When the results were segregated according to transplant type, CsA in liver and heart recipients was overestimated by RIA as compared with HPLC: slope = 1.202, r = 0.973 and slope = 1.1477, r = 0.983, respectively. Adult and pediatric CsA values were acceptable when RIA and HPLC were compared: slope = 1.0755, r = 0.977 and slope = 1.0563, r = 0.980, respectively. For six samples (four heart, two liver recipients) where HPLC and RIA values demonstrated wide discrepancies, repeat HPLC and analysis of eluate fractions gave CsA concentrations nearer values by the initial HPLC assay. We conclude that this RIA cannot be substituted for HPLC in the case of heart and liver recipients. The need for each laboratory to standardize the RIA is obvious.


The following articles in journals at HighWire Press have cited this article:


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 J Clin PharmacolHome page
M. Rheeders, M. Bouwer, and T. C. Goosen
Drug-drug interaction after single oral doses of the furanocoumarin methoxsalen and cyclosporine.
J. Clin. Pharmacol., July 1, 2006; 46(7): 768 - 775.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
J. M. Juenke, P. I. Brown, F. M. Urry, and G. A. McMillin
Specimen Dilution for C2 Monitoring with the Abbott TDxFLx Cyclosporine Monoclonal Whole Blood Assay
Clin. Chem., August 1, 2004; 50(8): 1430 - 1433.
[Full Text] [PDF]


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