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Clinical Chemistry, Vol 35, 1731-1734, Copyright © 1989 by American Association for Clinical Chemistry
RW Luxton, P Patel, G Keir and EJ Thompson
Department of Special Chemical Pathology, National Hospitals for Nervous Diseases, London, England.
By using a benzethonium chloride concentration 12-fold that described originally (Clin Chem 1979;25:1317-9), we developed a reliable method suitable for routine measurement of total protein in cerebrospinal fluid. Only 10 microL of sample is required. Reactivity to immunoglobulin G (IgG) and to albumin (Alb) is similar, as is necessary for specimens that can have very varied IgG/Alb ratios. The assay, performed in a microtiter plate for ease of use, has a between-batch coefficient of variation of 3.4% for a protein concentration of 450 mg/L. This contrasts with a dye-binding technique with Ponceau S, for which the CV was 9% at the same concentration.
The following articles in journals at HighWire Press have cited this article:
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  A. K. Sharma, A. E. Schultze, D. M. Cooper, R. Y. Reams, W. H. Jordan, and P. W. Snyder Development of a Percutaneous Cerebrospinal Fluid Collection Technique in F-344 Rats and Evaluation of Cell Counts and Total Protein Concentrations Toxicol Pathol, June 1, 2006; 34(4): 393 - 395. [Abstract] [Full Text] [PDF]
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 U. Demirkilic, E. Kuralay, H. Tatar, M. Kocakulak, C. Kocum, and H. Ayhan Investigation of Blood Compatibility and Determination of Clinical Performance of PMEA Coated and Flow Optimized Oxygenator Journal of Bioactive and Compatible Polymers, September 1, 2004; 19(5): 395 - 408. [Abstract] [PDF]
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 A. Kay, A. Petzold, M. Kerr, G. Keir, E. Thompson, and J. Nicoll Decreased Cerebrospinal Fluid Apolipoprotein E After Subarachnoid Hemorrhage: Correlation With Injury Severity and Clinical Outcome Stroke, March 1, 2003; 34(3): 637 - 642. [Abstract] [Full Text] [PDF]
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M. Kocakulak, C. Kocum, R. Saber, H. Ayhan, S. Gunaydin, T. Sari, Y. Zorlutuna, and N. Bingol Investigation of Blood Compatibility of PMEA Coated Extracorporeal Circuits Journal of Bioactive and Compatible Polymers, September 1, 2002; 17(5): 343 - 356. [Abstract] [PDF]
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 M. Winkler, B. Kemp, I. Clssen-Linke, D.-C. Fischer, S. Zaltinsi, J. Neulen, H. M. Beier, and W. Rath Estorgen Receptor {alpha} and Progesterone Receptor A and B Concentration and Localization the Lower Uterine Segment in Term Parturition Reproductive Sciences, July 1, 2002; 9(4): 226 - 232. [Abstract] [PDF]
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 E Caballero, M-E Drobnic, M-T Perez, J-M Manresa, A Ferrer, and R Orriols Anti-Pseudomonas aeruginosa antibody detection in patients with bronchiectasis without cystic fibrosis Thorax, September 1, 2001; 56(9): 669 - 674. [Abstract] [Full Text] [PDF]
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 H. L Brydon, G. Keir, E. J Thompson, R. Bayston, R. Hayward, and W. Harkness Protein adsorption to hydrocephalus shunt catheters: CSF protein adsorption J. Neurol. Neurosurg. Psychiatry, May 1, 1998; 64(5): 643 - 647. [Abstract] [Full Text] [PDF]
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 K. Barnard, R. Herold, H. Siemes, and M. Siegert Quantification of Cerebrospinal Fluid Proteins in Children by High-Resolution Agarose Gel Electrophoresis J Child Neurol, February 1, 1998; 13(2): 51 - 58. [Abstract] [PDF]
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 M. K. Sharief and R. Hentges Importance of Intrathecal Synthesis of IgD in Multiple Sclerosis: A Combined Clinical, Immunologic, and Magnetic Resonance Imaging Study Arch Neurol, October 1, 1991; 48(10): 1076 - 1079. [Abstract] [PDF]
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