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Clinical Chemistry 35: 1743-1746, 1989;
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Clinical Chemistry, Vol 35, 1743-1746, Copyright © 1989 by American Association for Clinical Chemistry

Murine monoclonal antibody adsorbed onto vinylidene fluoride floccules used to eliminate antibody interference in "sandwich"-type immunoassays

ES Newman, LA Moskie, RN Duggal, DM Goldenberg and HJ Hansen
Immunomedics Inc., Newark, NJ 07103.

We have previously reported that human anti-mouse IgG antibody (HAMA) can cause false-positive and false-negative results in "sandwich"-type monoclonal antibody (MAb) assays. To eliminate HAMA interference in "sandwich"-type MAb assays, we investigated the use of MAb on solid- phase, vinylidene fluoride floccules, which we have previously used as a solid-phase second antibody for RIA. The simple procedure effectively removes greater than 95% of HAMA from the most positive serum we have obtained from patients hyperimmunized to murine MAb, and it allows for accurate quantification of carcinoembryonic antigen. The solid-phase complex, added to blood, effectively removes HAMA and (or) "HAMA-type" heterophilic antibody from the sera or plasma.


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L. J. Kricka
Human Anti-Animal Antibody Interferences in Immunological Assays
Clin. Chem., July 1, 1999; 45(7): 942 - 956.
[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the American Association for Clinical Chemistry.