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Clinical Chemistry, Vol 35, 1949-1954, Copyright © 1989 by American Association for Clinical Chemistry
K Beever, J Bradbury, D Phillips, SM McLachlan, C Pegg, A Goral, W Overbeck, G Feifel and BR Smith
RSR Ltd., Pentwyn, Cardiff, U.K.
These highly sensitive assays are based on the interaction between thyroid autoantibodies and 125I-labeled autoantigens. Serum samples are incubated with labeled thyroid peroxidase (TPO) or thyroglobulin (Tg) to allow the formation of antibody-labeled antigen complexes. The complexes are then precipitated by addition of solid-phase Protein A. In the presence of high concentrations of TPO antibody or Tg antibody, more than 50% of the respective labeled antigen was precipitated, whereas only 1-2% was precipitated in the absence of autoantibody. Interassay CVs were 3.2% and 5.7%, respectively, for the anti-TPO and anti-Tg assays. There was no cross-reactivity between Tg antibody and TPO antibody. Results correlated highly significantly with results from other assay systems based on antigen-coated cells or plastic supports, but the assays described here were considerably more sensitive. Scatchard analysis of the assay data provided information on the affinity and serum concentration of TPO autoantibodies (ka approximately 10(9) L/mol and concentrations up to 1 g/L) and Tg autoantibodies (ka approximately 4 x 10(10) L/mol and concentrations up to 1 g/L). Overall, these assays provide a sensitive, precise, and convenient system for measuring and investigating the properties of thyroid autoantibodies.
The following articles in journals at HighWire Press have cited this article:
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  K. Zophel, G. Wunderlich, and J. Kotzerke A Highly Sensitive Thyroglobulin Assay Has Superior Diagnostic Sensitivity for Recurrence of Differentiated Thyroid Cancer in Patients Undergoing TSH Suppression J. Nucl. Med., March 1, 2006; 47(3): 552 - 553. [Full Text] [PDF]
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 F. Latrofa, M. Phillips, B. Rapoport, and S. M. McLachlan Human Monoclonal Thyroglobulin Autoantibodies: Epitopes and Immunoglobulin Genes J. Clin. Endocrinol. Metab., October 1, 2004; 89(10): 5116 - 5123. [Abstract] [Full Text] [PDF]
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 A. F. Muller, H. A. Drexhage, and A. Berghout Postpartum Thyroiditis and Autoimmune Thyroiditis in Women of Childbearing Age: Recent Insights and Consequences for Antenatal and Postnatal Care Endocr. Rev., October 1, 2001; 22(5): 605 - 630. [Abstract] [Full Text] [PDF]
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M. T. Kilbane, R. A. Ajjan, A. P. Weetman, R. Dwyer, E. W. M. McDermott, N. J. O’Higgins, and P. P. A. Smyth Tissue Iodine Content and Serum-Mediated 125I Uptake-Blocking Activity in Breast Cancer J. Clin. Endocrinol. Metab., March 1, 2000; 85(3): 1245 - 1250. [Abstract] [Full Text]
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 C. P. Mavragani, J. P. A. Ioannidis, A. G. Tzioufas, I. E. Hantoumi, and H. M. Moutsopoulos Recurrent pregnancy loss and autoantibody profile in autoimmune diseases Rheumatology, December 1, 1999; 38(12): 1228 - 1233. [Abstract] [Full Text] [PDF]
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 M. M. Kaplan Clinical Perspectives in the Diagnosis of Thyroid Disease Clin. Chem., August 1, 1999; 45(8): 1377 - 1383. [Abstract] [Full Text] [PDF]
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J. C. Jaume, J. Guo, Y. Wang, B. Rapoport, and S. M. McLachlan Cellular Thyroid Peroxidase (TPO), Unlike Purified TPO and Adjuvant, Induces Antibodies in Mice That Resemble Autoantibodies in Human Autoimmune Thyroid Disease J. Clin. Endocrinol. Metab., May 1, 1999; 84(5): 1651 - 1657. [Abstract] [Full Text]
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G. D. Chazenbalk, Y. Wang, J. Guo, J. S. Hutchison, D. Segal, J. C. Jaume, S. M. McLachlan, and B. Rapoport A Mouse Monoclonal Antibody to a Thyrotropin Receptor Ectodomain Variant Provides Insight into the Exquisite Antigenic Conformational Requirement, Epitopes and in Vivo Concentration of Human Autoantibodies J. Clin. Endocrinol. Metab., February 1, 1999; 84(2): 702 - 710. [Abstract] [Full Text]
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B. Rapoport, G. D. Chazenbalk, J. C. Jaume, and S. M. McLachlan The Thyrotropin (TSH)-Releasing Hormone Receptor: Interaction with TSH and Autoantibodies Endocr. Rev., December 1, 1998; 19(6): 673 - 716. [Abstract] [Full Text]
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S. Chen, J. Sawicka, L. Prentice, J. F. Sanders, H. Tanaka, V. Petersen, C. Betterle, M. Volpato, S. Roberts, M. Powell, et al. Analysis of Autoantibody Epitopes on Steroid 21-Hydroxylase Using a Panel of Monoclonal Antibodies J. Clin. Endocrinol. Metab., August 1, 1998; 83(8): 2977 - 2986. [Abstract] [Full Text]
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N. Despres and A. M. Grant Antibody interference in thyroid assays: a potential for clinical misinformation Clin. Chem., March 1, 1998; 44(3): 440 - 454. [Abstract] [Full Text] [PDF]
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H. Tanaka, M. S. Perez, M. Powell, J. F. Sanders, J. Sawicka, S. Chen, L. Prentice, T. Asawa, C. Betterle, M. Volpato, et al. Steroid 21-Hydroxylase Autoantibodies: Measurements with a New Immunoprecipitation Assay J. Clin. Endocrinol. Metab., May 1, 1997; 82(5): 1440 - 1446. [Abstract] [Full Text] [PDF]
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J. C. Jaume, A. Kakinuma, G. D. Chazenbalk, B. Rapoport, and S. M. McLachlan Thyrotropin Receptor Autoantibodies in Serum Are Present at Much Lower Levels Than Thyroid Peroxidase Autoantibodies: Analysis by Flow Cytometry J. Clin. Endocrinol. Metab., February 1, 1997; 82(2): 500 - 507. [Abstract] [Full Text] [PDF]
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