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Clinical Chemistry, Vol 35, 1957-1961, Copyright © 1989 by American Association for Clinical Chemistry
WA Ratcliffe, DA Heath, M Ryan and SR Jones
Wolfson Research Laboratories, Department of Clinical Chemistry, Queen Elizabeth Medical Centre, Birmingham, U.K.
The "N-tact" immunoradiometric assay (IRMA) from INCSTAR for parathyrin (PTH) in serum involves a 125I-labeled affinity-purified antiserum to PTH 1-34 and an affinity-purified antiserum to PTH 39-84, the latter bound to a polystyrene bead. The mean detection limit, determined in six consecutive assays, was 4 ng/L. The within-batch CV was less than 7% in the range 15 to 2135 ng/L. The between-batch CV was 11.7% and 5.3% at 30 and 371 ng/L, respectively. Serum PTH in 14 proven cases of primary hyperparathyroidism was 49-808 (median 111) ng/L, undetectable (less than 5 ng/L) in 10 cases of primary hypoparathyroidism and in 10 cases of hypercalcemia associated with malignancy, compared with 7-39 ng/L in 45 normal subjects. PTH was 9 to 19 ng/L in four patients with familial benign hypercalcemia. In 39 patients with renal failure, apparent concentrations were 14 to 857 (median 133) ng/L, but sera from these patients pre-diluted with zero standard did not parallel dilutions of the standard, PTH 1-84. PTH concentrations were not significantly decreased in blood or serum kept at 20 degrees C for up to 6 h. After successful removal of a parathyroid adenoma, the mean half-time for disappearance of PTH in vivo in five hyperparathyroid patients was 3.3 min.
The following articles in journals at HighWire Press have cited this article:
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  M. P Bjorkman, A. J Sorva, and R. S Tilvis Elevated serum parathyroid hormone predicts impaired survival prognosis in a general aged population Eur. J. Endocrinol., May 1, 2008; 158(5): 749 - 753. [Abstract] [Full Text] [PDF]
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 J.-C. Souberbielle, V. Fayol, C. Sault, E. Lawson-Body, A. Kahan, and C. Cormier Assay-Specific Decision Limits for Two New Automated Parathyroid Hormone and 25-Hydroxyvitamin D Assays Clin. Chem., February 1, 2005; 51(2): 395 - 400. [Abstract] [Full Text] [PDF]
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 S. J. Parikh, M. Edelman, G. I. Uwaifo, R. J. Freedman, M. Semega-Janneh, J. Reynolds, and J. A. Yanovski The Relationship between Obesity and Serum 1,25-Dihydroxy Vitamin D Concentrations in Healthy Adults J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1196 - 1199. [Abstract] [Full Text] [PDF]
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J.-C. Souberbielle, C. Cormier, C. Kindermans, P. Gao, T. Cantor, F. Forette, and E. E. Baulieu Vitamin D Status and Redefining Serum Parathyroid Hormone Reference Range in the Elderly J. Clin. Endocrinol. Metab., July 1, 2001; 86(7): 3086 - 3090. [Abstract] [Full Text] [PDF]
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J.-H. Brossard, R. Lepage, H. Cardinal, L. Roy, L. Rousseau, C. Dorais, and P. D'Amour Influence of Glomerular Filtration Rate on Non-(1-84) Parathyroid Hormone (PTH) Detected by Intact PTH Assays Clin. Chem., May 1, 2000; 46(5): 697 - 703. [Abstract] [Full Text] [PDF]
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H. Cardinal, J.-H. Brossard, L. Roy, R. Lepage, L. Rousseau, and P. D’Amour The Set Point of Parathyroid Hormone Stimulation by Calcium Is Normal in Progressive Renal Failure J. Clin. Endocrinol. Metab., November 1, 1998; 83(11): 3839 - 3844. [Abstract] [Full Text]
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R. Lepage, L. Roy, J.-H. Brossard, L. Rousseau, C. Dorais, C. Lazure, and P. D'Amour A non-(1–84) circulating parathyroid hormone (PTH) fragment interferes significantly with intact PTH commercial assay measurements in uremic samples Clin. Chem., April 1, 1998; 44(4): 805 - 809. [Abstract] [Full Text] [PDF]
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