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Clinical Chemistry 35: 1962-1964, 1989;
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Clinical Chemistry, Vol 35, 1962-1964, Copyright © 1989 by American Association for Clinical Chemistry

Microassay for screening newborns for galactosemia with use of a fluorometric microplate reader

A Yamaguchi, M Fukushi, Y Mizushima, Y Shimizu, N Takasugi, S Arashima and K Ohyanagi
Sapporo City Institute of Public Health, Japan.

We describe a microassay for measuring galactose (Gal) and galactose 1- phosphate (Gal-1-P) in dried blood spots. After a coupled enzyme reaction involving galactose dehydrogenase (GADH, EC 1.1.1.48) and alkaline phosphatase (AP, EC 3.1.3.1) in a microplate well, NADH fluorescence is measured by a highly sensitive fluorometric microplate reader, capable of rapid measurement of fluorescence (2 min per 96 samples). Within- and between-run CVs for measurements of Gal at 90 mg/L with Gal-1-P at 130 mg/L were both less than 5% (n = 8), and analytical recoveries for Gal at 90 mg/L and Gal-1-P at 130 mg/L were 98% and 92%, respectively. Five hundred dried blood-spot samples can be assayed within 2 h, with full calculation of results by an on-line microcomputer. This rapid and reliable assay system is very useful for the routine screening of newborns for galactosemia.


The following articles in journals at HighWire Press have cited this article:


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 Arch. Dis. Child. Fetal Neonatal Ed.Home page
K Murayama, H Nagasaka, K Tate, Y Ohsone, M Kanazawa, K Kobayashi, Y Kohno, and M Takayanagi
Significant correlations between the flow volume of patent ductus venosus and early neonatal liver function: possible involvement of patent ductus venosus in postnatal liver function
Arch. Dis. Child. Fetal Neonatal Ed., May 1, 2006; 91(3): F175 - F179.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
A. Fujimoto, Y. Okano, T. Miyagi, G. Isshiki, and T. Oura
Quantitative Beutler Test for Newborn Mass Screening of Galactosemia Using a Fluorometric Microplate Reader
Clin. Chem., June 1, 2000; 46(6): 806 - 810.
[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the American Association for Clinical Chemistry.