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Clinical Chemistry, Vol 35, 1975-1977, Copyright © 1989 by American Association for Clinical Chemistry
NR Musso, C Vergassola, A Pende and G Lotti
Cattedra Patologia Speciale Medica R, Ospedale San Martino, Universita' di Genova, Italy.
We present a method involving minor modifications of previous techniques, in which we use an ion-pair reversed-phase HPLC separation with three-electrode coulometry. Isocratic baseline separations can be obtained in 5 min. The sample preparation procedure, involving extraction with alumina at low temperature, allows good reproducibility (within-run and between-run CVs less than 10%) and improved sensitivity (less than 5 pg of each catecholamine per extract). This method allows approximately 70 low-cost plasma catecholamine analyses to be done in a working day.
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