Clinical Chemistry
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Clinical Chemistry 35: 1992-1994, 1989;
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Clinical Chemistry, Vol 35, 1992-1994, Copyright © 1989 by American Association for Clinical Chemistry

Test-strip method for measuring lactate in whole blood

N Shimojo, K Naka, C Nakajima, C Yoshikawa, K Okuda and K Okada
Department of Laboratory Medicine, Osaka City University Medical School, Japan.

We have developed a dry reagent strip system for measuring lactate in whole blood. The test strip contains lactate oxidase (no EC number assigned), horseradish peroxidase (EC 1.11.1.7), and N-ethyl-N-(2- hydroxy-3-sulfopropyl)-m-toluidine. The system is designed to measure with a reflectometer the color that developed in the test strip, although the lactate concentration can be estimated without the reflectometer. The between-run coefficients of variation for controls at three concentrations were 2.9-5.3%. The lactate concentrations in blood samples from healthy subjects before and after exercise correlated well (r = 0.97) with the results measured by the comparison method with the use of lactate oxidase. This dry reagent strip system provides a convenient and rapid test for measuring blood lactate in clinical and sports medicine.




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Copyright © 1989 by the American Association for Clinical Chemistry.