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Clinical Chemistry 36: 110-112, 1990;
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Clinical Chemistry, Vol 36, 110-112, Copyright © 1990 by American Association for Clinical Chemistry

Fluorescence polarization immunoassay of urinary vanillylmandelic acid

GW Mellor and G Gallacher
Department of Chemical Pathology, St. Bartholomew's Hospital, West Smithfield, London, U.K.

This rapid fluorescence polarization immunoassay for urinary vanillylmandelic acid (VMA) involves use of our previously described antiserum and label and the program for 5-hydroxyindoleacetic acid in the Abbott TDx fluorimeter. Urine samples were measured directly, without pretreatment. The minimum detectable concentration was 0.3 mg/L, and the range of the standard curve was 0.3-200.0 mg/L. Precision, analytical recovery, and correlation of results with those by the Pisano method (Clin Chim Acta 1962;7:285-91) were all satisfactory. With this procedure one can determine the VMA concentration in 10 urine sample in 22 min. This is the first report of a clinical immunoassay for VMA and should greatly simplify screening for neural crest tumors.


The following articles in journals at HighWire Press have cited this article:


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F. Taran, Y. Frobert, C. Creminon, J. Grassi, D. Olichon, C. Mioskowski, and P. Pradelles
Competitive enzyme immunoassay with monoclonal antibody for homovanillic acid measurement in human urine samples
Clin. Chem., February 1, 1997; 43(2): 363 - 368.
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