Clinical Chemistry, Vol 36, 123-125, Copyright © 1990 by American Association for Clinical Chemistry

Oligoclonal bands in cerebrospinal fluid detected by PhastSystem isoelectric focusing

I Wybo, M Van Blerk, R Malfait, P Goubert and F Gorus
Department of Clinical Chemistry, Academic Hospital, Vrije Universiteit Brussel, Belgium.

Pharmacia's "PhastSystem" for semi-automated isoelectric focusing (IEF) in thin precast polyacrylamide gels (PAGE) was found to be as sensitive as high-resolution protein electrophoresis (HRPE) in agarose gels and conventional PAGE-IEF for detection of oligoclonal banding (OB) in concentrated cerebrospinal fluid (CSF) samples. Both PhastSystem IEF and HRPE revealed OB in CSF from eight of nine multiple sclerosis patients and four of 10 patients with various types of infection of the central nervous system as opposed to only two of 70 patients with miscellaneous neuropsychiatric disorders. The PhastSystem also frequently detected OB in silver-stained, unconcentrated CSF from patients with multiple sclerosis.

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