Clinical Chemistry
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Clinical Chemistry 36: 76-80, 1990;
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Clinical Chemistry, Vol 36, 76-80, Copyright © 1990 by American Association for Clinical Chemistry

A new enzyme immunoassay for prolactin in serum or plasma

R Babiel, P Willnow, M Baer, M van Gent and V Ehrhardt
Boehringer Mannheim GmbH, Biochemical Research Centre, Tutzing, F.R.G.

This enzyme immunoassay (EIA) of human prolactin (hPRL) involves incubation of sample and anti-hPRL antibodies conjugated to horseradish peroxidase (EC 1.11.1.7) in tubes coated with a second antibody to hPRL. The test can be performed within 60 min. No reaction of the antibodies with human placental lactogen and human somatotropin is detectable. The presence of detergent allows assay of both serum and plasma. Precision was improved by including polyethylene glycol in the reaction mixture. To optimize analytical recovery, we added protease inhibitor. Assay of the EIA standards shows good correlation with results for World Health Organization reference preparations. The measurable range is 1 to 400 micrograms/L. Intra- and interassay CVs are about 5%. Comparisons with two RIAs and two other EIAs show reasonably good correlations. The components of our EIA are stable for 18 months.




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Copyright © 1990 by the American Association for Clinical Chemistry.