Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 36: 1769-1773, 1990;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Worthman, C. M.
Right arrow Articles by Hofman, L. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Worthman, C. M.
Right arrow Articles by Hofman, L. F.

Clinical Chemistry, Vol 36, 1769-1773, Copyright © 1990 by American Association for Clinical Chemistry

Sensitive salivary estradiol assay for monitoring ovarian function

CM Worthman, JF Stallings and LF Hofman
Department of Anthropology, Emory University, Atlanta, GA 30322.

Measurement of steroids in saliva has excited interest because of the numerous potential clinical applications; noninvasive, convenient sampling; and apparently accurate reflection of the concentrations of physiologically active unbound steroid in the circulation. Although assays of saliva for several steroid hormones are available and widely used, assays for salivary estradiol are not, primarily because of methodological limitations. By modifying a commercially available kit for serum estradiol, our laboratory has developed a procedure that is sensitive, highly specific, and reliable for measuring salivary estradiol. Assay sensitivity is 0.5 fmol (0.14 pg; sample concentration 1.3 pmol/L) with a mean interassay CV of 10.8% at low concentrations. Clinical studies showed that values for serum and saliva are highly correlated (P less than 0.001), and demonstrated reliable detection of estradiol peaks during normal ovulatory cycles in serial samples from 15 women. Salivary estradiol peaked at 5.4 (SD 1.9) pmol/L on cycle day 14.4 (SD 3.2), 1.2 (SD 0.8) days before ovulation detected by ultrasound. This assay may be particularly helpful in investigating ovarian function and free estradiol in women at various stages of the reproductive cycle.


The following articles in journals at HighWire Press have cited this article:


Home page
 EndocrinologyHome page
J. B. Becker, A. P. Arnold, K. J. Berkley, J. D. Blaustein, L. A. Eckel, E. Hampson, J. P. Herman, S. Marts, W. Sadee, M. Steiner, et al.
Strategies and Methods for Research on Sex Differences in Brain and Behavior
Endocrinology, April 1, 2005; 146(4): 1650 - 1673.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
R. E. Nelson, S. K. Grebe, D. J. O'Kane, and R. J. Singh
Liquid Chromatography-Tandem Mass Spectrometry Assay for Simultaneous Measurement of Estradiol and Estrone in Human Plasma
Clin. Chem., February 1, 2004; 50(2): 373 - 384.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. B. Patisaul, M. Melby, P. L. Whitten, and L. J. Young
Genistein Affects ER{beta}- But Not ER{alpha}-Dependent Gene Expression in the Hypothalamus
Endocrinology, June 1, 2002; 143(6): 2189 - 2197.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. B. Patisaul, M. Dindo, P. L. Whitten, and L. J. Young
Soy Isoflavone Supplements Antagonize Reproductive Behavior and Estrogen Receptor {{alpha}}- and {beta}-Dependent Gene Expression in the Brain
Endocrinology, July 1, 2001; 142(7): 2946 - 2952.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
L. F. Hofman
Human Saliva as a Diagnostic Specimen
J. Nutr., May 1, 2001; 131(5): 1621S - 1625.
[Abstract] [Full Text]

Home page
 Clin. Chem.Home page
K. Tamate, M. Charleton, J. P. Gosling, D. Egan, M. Ishikawa, P. F. Fottrell, and M. M. Kane
Direct colorimetric monoclonal antibody enzyme immunoassay for estradiol-17{beta} in saliva
Clin. Chem., July 1, 1997; 43(7): 1159 - 1164.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by the American Association for Clinical Chemistry.