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Clinical Chemistry 36: 1793-1799, 1990;
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Clinical Chemistry, Vol 36, 1793-1799, Copyright © 1990 by American Association for Clinical Chemistry

Enzyme immunoassay of human placental and germ-cell alkaline phosphatase in serum

PG Hendrix, MF Hoylaerts, EJ Nouwen and ME De Broe
Department of Nephrology-Hypertension, University of Antwerp, Belgium.

Placental alkaline phosphatase (placental ALP, PLAP) and germ-cell ALP (GCAP, also known as placental-like ALP), expressed in gonadal cancer tissues, are potential tumor markers. Four monoclonal antibodies, raised against PLAP and recognizing different epitopes, were selected to study the influence of the following variables on the accuracy of PLAP and GCAP measurement: phenotype, molecular form, and glycation pattern of PLAP and GCAP; incubation temperature; and interferences by serum during immunobinding. Nine GCAP phenotypes were identified, interacting with each antibody at a lower affinity than was seen for the more common PLAP phenotypes. Antibody affinity is higher for the free hydrophilic dimeric forms of PLAP and GCAP, and is not influenced by the degree of glycation. In serum or tissue extracts, measurement of PLAP or GCAP is most nearly accurate when immunoincubations are performed at 37 degrees C, with use of antibodies 327 and 7E8, respectively. In addition, correct measurements are achieved only when, during immunobinding, serum is incubated with an equal volume of deoxycholate (9 g/L final concentration).


The following articles in journals at HighWire Press have cited this article:


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 J. Biol. Chem.Home page
M.-H. Le Du and J. L. Millan
Structural Evidence of Functional Divergence in Human Alkaline Phosphatases
J. Biol. Chem., December 13, 2002; 277(51): 49808 - 49814.
[Abstract] [Full Text] [PDF]

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 J. Biol. Chem.Home page
M. F. Hoylaerts, T. Manes, and J. L. Millan
Mammalian Alkaline Phosphatases Are Allosteric Enzymes
J. Biol. Chem., September 5, 1997; 272(36): 22781 - 22787.
[Abstract] [Full Text] [PDF]


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