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Clinical Chemistry 36: 1834-1837, 1990;
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Clinical Chemistry, Vol 36, 1834-1837, Copyright © 1990 by American Association for Clinical Chemistry

Simultaneous determination of acidic 3,4-dihydroxyphenylalanine metabolites and 5-hydroxyindole-3-acetic acid in urine by high- performance liquid chromatography

AE Stroomer, H Overmars, NG Abeling and AH van Gennip
Department of Pediatrics, University Hospital, Amsterdam, The Netherlands.

We describe a simple and rapid quantitative method for the simultaneous determination of 3,4-dihydroxyphenylalanine acid metabolites and 5- hydroxyindole-3-acetic acid. After solvent extraction from acidified urine, the acids are analyzed by reversed-phase high-performance liquid chromatography. For detection and quantification, we used a fluorescence detector in combination with an amperometric detector to obtain a high degree of specificity. Sample preparation and chromatographic analysis can be completed within an hour. Results by the method correlate well with those by a previously used gas- chromatographic method, but the new method is faster and avoids the need for derivatization.


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J. M. Zuetenhorst, C. M. Korse, J. M.G. Bonfrer, E. Peter, C. B.H.W. Lamers, and B. G. Taal
Daily Cyclic Changes in the Urinary Excretion of 5-Hydroxyindoleacetic Acid in Patients with Carcinoid Tumors
Clin. Chem., September 1, 2004; 50(9): 1634 - 1639.
[Abstract] [Full Text] [PDF]


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