Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 36: 1871-1874, 1990;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hill, J. S.
Right arrow Articles by Pritchard, P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hill, J. S.
Right arrow Articles by Pritchard, P. H.

Clinical Chemistry, Vol 36, 1871-1874, Copyright © 1990 by American Association for Clinical Chemistry

Improved phenotyping of apolipoprotein E: application to population frequency distribution

JS Hill and PH Pritchard
Department of Pathology, University of British Columbia, Vancouver, Canada.

A simple procedure for phenotyping apolipoprotein (apo) E directly from plasma has been developed for use in the lipid clinic laboratory. In this new method, 10 microL of serum or plasma is pretreated with neuraminidase (EC 3.2.1.18), which removes the sialic acid residues from apo E and eliminates additional bands, thereby ensuring correct phenotype assignment. After a rapid delipidation step, the samples are focused in vertical polyacrylamide mini-slab gels and immunoblotted with a polyclonal goat anti-apo E antibody, followed by a Protein G- peroxidase conjugate. The accuracy of this method was confirmed by comparison with the established procedure of phenotyping by isoelectric focusing of delipidated very-low-density lipoprotein. In addition, sera from 203 subjects from Vancouver, selected without conscious bias, were used to determine the local distribution of the apo E alleles. We estimate that the relative frequencies of apo E alleles epsilon 2, epsilon 3, and epsilon 4 in this population are 0.086, 0.761, and 0.153, respectively. The speed and convenience of using minigels make this procedure ideal for clinical laboratory applications and large population studies.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Lipid Res.Home page
L. Krimbou, M. Marcil, H. Chiba, and J. Genest Jr.
Structural and functional properties of human plasma high density-sized lipoprotein containing only apoE particles
J. Lipid Res., May 1, 2003; 44(5): 884 - 892.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S. Lussier-Cacan, A. Bolduc, M. Xhignesse, T. Niyonsenga, and C. F. Sing
Impact of Alcohol Intake on Measures of Lipid Metabolism Depends on Context Defined by Gender, Body Mass Index, Cigarette Smoking, and Apolipoprotein E Genotype
Arterioscler. Thromb. Vasc. Biol., May 1, 2002; 22(5): 824 - 831.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
C. Marcoux, P. N. Hopkins, T. Wang, E. T. Leary, K. Nakajima, J. Davignon, and J. S. Cohn
Remnant-like particle cholesterol and triglyceride levels of hypertriglyceridemic patients in the fed and fasted state
J. Lipid Res., September 1, 2000; 41(9): 1428 - 1436.
[Abstract] [Full Text]

Home page
 J. Lipid Res.Home page
C. Marcoux, M. Tremblay, K. Nakajima, J. Davignon, and J. S. Cohn
Characterization of remnant-like particles isolated by immunoaffinity gel from the plasma of type III and type IV hyperlipoproteinemic patients
J. Lipid Res., April 1, 1999; 40(4): 636 - 647.
[Abstract] [Full Text]

Home page
 J. Lipid Res.Home page
L. Krimbou, M. Tremblay, J. Davignon, and J. S. Cohn
Association of apolipoprotein E with {alpha}2-macroglobulin in human plasma
J. Lipid Res., December 1, 1998; 39(12): 2373 - 2386.
[Abstract] [Full Text]

Home page
 J. Lipid Res.Home page
L. Krimbou, M. Tremblay, H. Jacques, J. Davignon, and J. S. Cohn
In vitro factors affecting the concentration of gamma-LpE ({gamma}-LpE) in human plasma
J. Lipid Res., April 1, 1998; 39(4): 861 - 872.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. S. Cohn, L.-M. Giroux, L.-J. Fortin, and J. Davignon
Prevalence of Double Pre-Beta Lipoproteinemia in Hyperlipidemic Patients Is Influenced by Gender, Menopausal Status, and ApoE Phenotype
Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 2630 - 2637.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. S. Cohn, M. Tremblay, M. Amiot, D. Bouthillier, M. Roy, J. Genest Jr, and J. Davignon
Plasma Concentration of Apolipoprotein E in Intermediate-Sized Remnant-Like Lipoproteins in Normolipidemic and Hyperlipidemic Subjects
Arterioscler. Thromb. Vasc. Biol., January 1, 1996; 16(1): 149 - 159.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
L. Krimbou, M. Marcil, J. Davignon, and J. Genest Jr.
Interaction of Lecithin:Cholesterol Acyltransferase (LCAT){middle dot}alpha 2-Macroglobulin Complex with Low Density Lipoprotein Receptor-related Protein (LRP). EVIDENCE FOR AN alpha 2-MACROGLOBULIN/LRP RECEPTOR-MEDIATED SYSTEM PARTICIPATING IN LCAT CLEARANCE
J. Biol. Chem., August 24, 2001; 276(35): 33241 - 33248.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by the American Association for Clinical Chemistry.